- Rothera’s test
- Gerhardt’s test
- Lang’s test
- Lindeman’s test
- Han’s test
- Tablet test
Principle of Rothera’s Test
Acetoacetic acid and acetone react with an alkaline solution of sodium nitroprusside to form a purple-colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected.
Requirements
- Urine specimen
- Test tubes
- Rothera’s powder:
Sodium nitroprusside = 0.75 gm
Ammonium sulphate = 20gm
Mix and pulverize. - Liquor Ammonia (Ammonium hydroxide)
The procedure of Rothera’s Test
- Transfer about 5 ml of urine to a test tube.
- Add 1 gm of Rothera’s powder mixture and mix well.
- Layer over the urine 1-2 ml of concentrated ammonium hydroxide.
- Observe the pink-purple ring at the interface.
Observations and Results
- Immediate formation of purple permanganate colored ring at the interface: Ketone bodies present (Positive)
- No formation of purple permanganate colored ring at the interface: ketone bodies absent (Negative)
Grade the result according to intensity of the formation of colored ring as Trace, +, ++, +++ or ++++.
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